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藥靶細(xì)胞株 > 腫瘤免疫細(xì)胞株 > CBP74091TLR9/NFkB-Luc/HEK293
- 詳細(xì)內(nèi)容
| I. Background | |
Toll 樣受體(TLR)是一種模式識(shí)別受體(PRR),高度保守,可識(shí)別各種類型的微 生物病原體相關(guān)模式分子(PAMP)。在人類中發(fā)現(xiàn)了 10 個(gè) Toll 樣受體家族(TLR1-10) 成員,在小鼠中發(fā)現(xiàn)了 12 個(gè)成員(TLR1-9、TLR11 and TLR13)。TLR1、TLR2、TLR4、 TLR5 和 TLR6 分布在細(xì)胞膜表面;TLR3、TLR7、TLR8、TLR9 和 TLR10 分布在細(xì)胞內(nèi) 涵體膜表面(TLR3 識(shí)別 dsRNA,TLR9 識(shí)別 dsDNA)。TLRs 下游信號(hào)的激活主要依賴于 兩類轉(zhuǎn)錄因子:NF-κB 和干擾調(diào)節(jié)因子(IRFs),它們主要誘導(dǎo)促炎細(xì)胞因子和 I 型干擾 素(IFN)的產(chǎn)生。 | |
| II. Description | |
TLR9 NFκB-Luc HEK293 報(bào)告基因藥靶模型很好的模擬了體內(nèi) TLR9 的信號(hào)轉(zhuǎn)導(dǎo)過程, 原理見下圖所示。
| |
| II. Introduction | |
| Host Cell: | HEK293 |
| Expressed gene: | TLR9、NFκB-Luc |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): | TLR, TLR-9, tlr, nfkb, nf-kb |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | MEM + 10% Foetal Bovine Serum (FBS)+ 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) +1μg/ml puromycin+100μg/ml Hygromycin B |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| III. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Embryonic kidney |
| Disease: | Normal |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |

Figure 2.Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001(we strongly suggest to purchase from Cobioer). Dose Response of ODN 2006 in TLR9 NFκB-Luc HEK293(C3).





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