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Cell Line Name：

BCR-ABL1 [Q252H/T315I] /BaF3  

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

ABL1, ABL proto-oncogene 1, non-receptor tyrosine kinase, plays a role in regulation of cell growth through ERK5, Rac/JNK, and Stat1/3 pathways (PMID: 10373409). Fusion proteins of BCR-ABL1 resulting in constitutive kinase activity have been reported in various cancers (PMID: 18851712, PMID: 30125955) and a number of Abl1 mutations in the context of BCR-ABL1 have been demonstrated to confer drug resistance (PMID: 24456693).

1.WB of BCR-ABL1 [Q252H/T315I] /BaF3  

2. Sanger of BCR-ABL1 [Q252H/T315I] /BaF3

Figure 2. BCR-ABL1 [Q252H/T315I] /BaF3 Fusion

Figure 3. BCR-ABL1 [Q252H/T315I] /BaF3 Q252H Mutation

Figure 4. BCR-ABL1 [Q252H/T315I] /BaF3 T315I Mutation

3. Anti-proliferation assay

Figure 5 .CTG Proliferation Assay of BaF3 BCR-ABL1 T315I Q252H Cells (C1).